Confocal Microscopy: Difference between revisions

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A [https://en.wikipedia.org/wiki/Confocal_microscopy Confocal Microscopy] is an optical imaging technique for increasing optical resolution and contrast of a micrograph by means of using a spatial pinhole to block out-of-focus light in image formation.
==Introduction==
==Introduction==
A [https://en.wikipedia.org/wiki/Confocal_microscopy Confocal Microscopy] or Confocal Laser Scanning Microscopy (CLSM) uses pinhole to block out all out of focus light to enhance optical resolution, very different from traditional wide-field fluorescence microscopes. To offset the block of out of focus lights, the light intensity is detected by a photomultiplier tube or avalanche photodiode, which transforms the light signal into an electrical one. We will try to build a Setup like this to enhance optical resolution and maybe get profile information about the sample.
A Confocal microscopy use pinhole to block out all out of focus light to enhance optical resolution, very different from traditional wide-field fluorescence microscopes. To offset the block of out of focus lights, the light intensity is detected by a photomultiplier tube or avalanche photodiode, which transforms the light signal into an electrical one. We will try to build a Setup like this to enhance optical resolution and maybe get profile information about the sample.


==Team Members==
==Team Members==

Revision as of 06:02, 8 February 2022

A Confocal Microscopy is an optical imaging technique for increasing optical resolution and contrast of a micrograph by means of using a spatial pinhole to block out-of-focus light in image formation.

Introduction

A Confocal microscopy use pinhole to block out all out of focus light to enhance optical resolution, very different from traditional wide-field fluorescence microscopes. To offset the block of out of focus lights, the light intensity is detected by a photomultiplier tube or avalanche photodiode, which transforms the light signal into an electrical one. We will try to build a Setup like this to enhance optical resolution and maybe get profile information about the sample.

Team Members

Wang Tingyu, Xue Rui, Yang Hengxing

Lab Location

S12-01-10/11 Nanomaterials Research Lab