Confocal Microscopy: Difference between revisions
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Created page with "==Introduction== A Confocal Microscopy or Confocal Laser Scanning Microscopy (CLSM) uses pinhole to block out all out of focus light to enhance optical resolution, very different from traditional wide-field fluorescence microscopes. To offset the block of out of focus lights, the light intensity is detected by a photomultiplier tube or avalanche photodiode, which transforms the light signal into an electrical one. We will try to build a Setup like this to enhance optical..." |
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==Introduction== | ==Introduction== | ||
A Confocal Microscopy or Confocal Laser Scanning Microscopy (CLSM) uses pinhole to block out all out of focus light to enhance optical resolution, very different from traditional wide-field fluorescence microscopes. To offset the block of out of focus lights, the light intensity is detected by a photomultiplier tube or avalanche photodiode, which transforms the light signal into an electrical one. We will try to build a Setup like this to enhance optical resolution and maybe get profile information about the sample. | A Confocal Microscopy or Confocal Laser Scanning Microscopy (CLSM) uses pinhole to block out all out of focus light to enhance optical resolution, very different from traditional wide-field fluorescence microscopes. To offset the block of out of focus lights, the light intensity is detected by a photomultiplier tube or avalanche photodiode, which transforms the light signal into an electrical one. We will try to build a Setup like this to enhance optical resolution and maybe get profile information about the sample. | ||
==Team Members== | |||
Wang Tingyu, Xue Rui, Yang Hengxing | |||
Revision as of 08:07, 25 January 2022
Introduction
A Confocal Microscopy or Confocal Laser Scanning Microscopy (CLSM) uses pinhole to block out all out of focus light to enhance optical resolution, very different from traditional wide-field fluorescence microscopes. To offset the block of out of focus lights, the light intensity is detected by a photomultiplier tube or avalanche photodiode, which transforms the light signal into an electrical one. We will try to build a Setup like this to enhance optical resolution and maybe get profile information about the sample.
Team Members
Wang Tingyu, Xue Rui, Yang Hengxing