Confocal Microscopy

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Introduction

A Confocal Microscopy is an optical imaging technique for increasing optical resolution and contrast of a micrograph. It uses pinhole to block out all out of focus light to enhance optical resolution, very different from traditional wide-field fluorescence microscopes. To offset the block of out of focus lights, the light intensity is detected by a photomultiplier tube or avalanche photodiode, which transforms the light signal into an electrical one. We will try to build a Setup like this to enhance optical resolution and maybe get profile information about the sample.

Team Members

Wang Tingyu, Xue Rui, Yang Hengxing

Principles of confocal microscopy

Set up

Figure 2. Optical path diagram of confocal microscopy

Lab Location

S12-01-10/11 Nanomaterials Research Lab